WIZ™ HOTaq DNA Polymerase is hot-start Taq DNA Polymerase, which is a modified form of Taq DNA Polymerase. WIZ™ HOTaq DNA Polymerase is provided in an inactive state and has minimum enzymatic activity at ambient temperatures. This prevents the formation of misprimed products during reaction setup and the first denaturation step, leading to high PCR specific. it is suitable for dignostic reaction without minerband. The enzyme is a highly processive 5’-> 3’ DNA polymerase that lacks 3’-> 5’ exonuclease activity. Each lot of WIZ™ HOTaq DNA polymerase is tested for PCR amplification. The enzyme is supplied at 5 units/ul and comes with an optimized 10X PCR reaction buffer.
Contents - WIZ™ HOTaq DNA Polymerase (5U/μl) - 10X Reaction Buffer - dNTP mix (2.5mM)
Characteristics - Error rate : 2.4 X 10-5 - Thermal stability : half life of 40 min at 95°C - A-tail formation at 3’ ends of amplified duplex DNA. - No activity at temperatures lower than 75°C.
- Hot-start PCR - High specific amplification of DNA fragments shorter than 3kb. - Amplification of cDNA and genomic DNA. - Amplification of template DNA with secondary or higher-ordered structure that is resistant to PCR amplification - Primer extension - Multiplex PCR
Shipping and Storage Conditions - Store all components at -20ºC in a non-frost-free freezer. - Shipping at room temperature has no detrimental effects on the quality of this reagent.
WiZ™ Taq DNA Polymerase is high fidelity Taq DNA Polymerase,
WIZ™ HOTaq DNA Polymerase is hot-start Taq DNA Polymerase, w
WIZ™ Pfu DNA Polymerase is a thermostable enzyme derived fro
WIZ™ Taq PCR 2X Master is an economical, highly efficient an
WIZ™ HOTaq PCR 2X Master is ready-to-use Hot-start PCR pre-m
WIZ™ Pfu PCR 2X Master is ready-to-use Pfu DNA polymerase pr
WIZ™ Taq PCR 2X Premix combines all the reagents necessary f
WIZ™ HOTaq PCR 2X Premix combines all the reagents necessary
WIZ™ Taq PCR FD Premix combines all the reagents necessary f
WIZ™ HOTaq PCR FD Premix combines all the reagents necessary
The M-MLV RTase (RNase H-) is a genetically modified M-MLV R
WIZ™ RT-PCR 2X Master combines all the reagents necessary fo