WIZ™ HOTaq PCR 2X Premix combines all the reagents necessary for successful hot-start PCR in a convenient individually aliquoted in single-tube. WIZ™ HOTaq PCR 2X Premix is an optimized, economical, highly efficient and ready-to-use hot-start PCR premix which can amplify templates of up to 5 kb. The PCR mixture of hot-start Taq DNA Polymerase, PCR buffer, MgCl2 and dNTP’s. This HOTaq PCR 2X Premix contains all components for PCR, except DNA template and primers. The mixture is suitable for amplification of most of the DNA templates. Tested for absence of endo nucleases & exo nucleases and is also tested for amplification of single gene copy. WIZ™ HOTaq PCR 2X Premix yields excellent and consistent results in routine PCR reactions as well as high-throughput PCR genotyping, colony PCR, RT-PCR and PCR cloning. PCR reactions can be directly loaded onto an agarose gel without the additional need of loading buffer and dyes.
Contents - WIZ™ HOTaq PCR 2X Premix ( in 0.2ml 8-strip PCR tube ) : Containing Taq DNA Polymerase (0.2 unit/µl), Taq buffer (3 mM MgCl2), dNTP mixture (0.4 mM each), Stabilizer and loading dyes.
Characteristics - Single-tube convenience. - Well-suited to analysis of a large number of samples. - Including loading dye for easy gel electrophoresis. - A-tail formation at 3’ ends of amplified duplex DNA.
- Hot-start PCR - High through-put PCR - Routine diagnostic PCR requiring high reproducibility - DNA sequencing template preparation
Shipping and Storage Conditions - Stable up to 2 years at -20ºC or 6 months at 4ºC (Storage at -20ºC is recommended). - Shipping at room temperature has no detrimental effects on the quality of this reagent.
WiZ™ Taq DNA Polymerase is high fidelity Taq DNA Polymerase,
WIZ™ HOTaq DNA Polymerase is hot-start Taq DNA Polymerase, w
WIZ™ Pfu DNA Polymerase is a thermostable enzyme derived fro
WIZ™ Taq PCR 2X Master is an economical, highly efficient an
WIZ™ HOTaq PCR 2X Master is ready-to-use Hot-start PCR pre-m
WIZ™ Pfu PCR 2X Master is ready-to-use Pfu DNA polymerase pr
WIZ™ Taq PCR 2X Premix combines all the reagents necessary f
WIZ™ HOTaq PCR 2X Premix combines all the reagents necessary
WIZ™ Taq PCR FD Premix combines all the reagents necessary f
WIZ™ HOTaq PCR FD Premix combines all the reagents necessary
The M-MLV RTase (RNase H-) is a genetically modified M-MLV R
WIZ™ RT-PCR 2X Master combines all the reagents necessary fo