WIZ™ HOTaq PCR FD Premix combines all the reagents necessary for successful hot-start PCR in a convenient individually aliquoted and lyophiliezed in single-tube. WIZ™ HOTaq PCR FD Premix is an optimized, economical, highly efficient and ready-to-use hot-start PCR premix which can amplify templates of up to 5 kb. The PCR mixture of hot-start Taq DNA Polymerase, PCR buffer, MgCl2 and dNTP’s. This HOTaq PCR FD Premix contains all components for PCR, except DNA template and primers. The mixture is suitable for amplification of most of the DNA templates. Tested for absence of endo-nucleases & exo-nucleases and is also tested for amplification of single gene copy. WIZ™ HOTaq PCR FD Premix yields excellent and consistent results in routine PCR reactions as well as high-throughput PCR genotyping, colony PCR, RT-PCR and PCR cloning. PCR reactions can be directly loaded onto an agarose gel without the additional need of loading buffer and dyes.
Contents - WIZ™ HOTaq PCR FD Premix ( in 0.2ml 8-strip PCR tube)
Characteristics - Simplicity : Readily soluble upon addition of primers and template DNA and D.W. There is no need additional step to solubilize by mixing. - Convenience : Dry pellets are tightly bound to the bottom of the PCR tubes; easy to transport and handle. - Stability : The presence of a unique stabilizer ensures great stability of the PCR DNA polymerase even at room temperature (up to 2 months). - Easy for gel electrophoresis : Containing loading dye.
- Hot-start PCR - High through-put PCR - Routine diagnostic PCR requiring high reproducibility - DNA sequencing template preparation
Shipping and Storage Conditions - Stable up to 2 years at -20ºC or 6 months at 4ºC or 2 months at room temperature(Storage at -20ºC is recommended). - Shipping at room temperature has no detrimental effects on the quality of this reagent.
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