WIZ RT-PCR 2X Premix

Product Origin:
Korea

Description


WIZ™ RT-PCR 2X Premix combines all the reagents necessary for successful routine RT-PCR in a convenient individually aliquot in single-tube, one-step format. The premixed formulation saves time and reduces potential contaminating errors by eliminating several pipetting steps. WIZ™ RT-PCR 2X Premix is specifically designed to amplify targets up to 1.0 kb and is free of detectable nonspecific nucleases. An enhanced buffer allows for RT reaction temperatures up to 50°C. This can improve detection of more difficult targets as higher RT temperatures reduce nonspecific priming and facilitate melting of RNA secondary structures. In addition, the RT-PCR 2X Premix includes the recombinant RNase H- form of M-MLV Reverse Transcriptase, which has been shown to improve the sensitivity for certain targets. The RNase H activity degrades the RNA part of the cDNA/RNA hybrid following reverse transcription, which may prevent its inhibition during subsequent PCR steps. RT-PCR converts and amplifies single-stranded RNA template yielding doublestranded DNA product. In the RT step, reverse transcriptase synthesizes single-stranded DNA molecules complementary to the RNA template (first-strand cDNA). During the PCR step, a thermostable DNA polymerase first synthesizes second-strand DNA complementary to the first-strand cDNA molecules. This generates a double-stranded DNA template which is exponentially amplified in subsequent rounds of thermal cycling.

Product Feature
Contents - WIZ™ RT-PCR 2X Premix (20 µl reaction, in 0.2ml 8-strip PCR tube)

Product Specification/Models
Characteristics - Single-tube convenience - Synthesis of long cDNA - Excellent sensitivity

Application
- Routine and direct RT-PCR amplification of RNA templates - Multiple band detection or genotyping - Virus detection - Gene-expression analysis - cDNA library construction - 3 and 5 RACE, RT-PCR

Other Information
WZ240001

Payment
T/T

Delivery
1~4 weeks

Origin
Korea

Minimum Order
>1

Packaging
96 tubes

Samples
Free

Remarks
Shipping and Storage Conditions - Store all components at -20ºC in a non-frost-free freezer. - Shipping at room temperature has no detrimental effects on the quality of this reagent.

WIZBIO SOLUTIONS.

Address:
Gyeonggi-Do
Phone:
82-707-013-5066
Business Type:
Exporter/
Business Activity:
Manufacturer/Retailer/Wholesaler/
Target Markets:
World Wide
Year established
5/2010
Contact:
Davdi Lee

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